rabbit anti-mouse nestin polyclonal antibody  (US Biological Life Sciences)

Journal: The Journal of Neuroscience

Article Title: Induction of Neuregulin Signaling in Mouse Schwann Cells In Vivo Mimics Responses to Denervation

doi: 10.1523/JNEUROSCI.1086-06.2006

Figure Lengend Snippet: Induction of active ErbB2 results in SC mitosis. A, B, Two views of an NMJ in a postnatal day 7 soleus muscle after 3 d of Dox induction; BrdU was given by intraperitoneal injections during the 3 d of induction. Arrow in B indicates one BrdU+ SC. C, D, Two views of an NMJ in an adult sternomastoid muscle after 10 d of Dox induction; BrdU was given in the drinking water during the last 3 d of induction. A polyclonal antibody to S100 was used to identify SCs (A, C), and monoclonal antibody to BrdU was used to identify nuclei that had incorporated BrdU (B, D). In both the neonatal and the adult muscle, the induction of mutant ErbB2 resulted in BrdU-labeled SC nuclei. BrdU-labeled cells are indicated by arrows in C and D. Scale bar, 30 μm.

Article Snippet: Reactive Schwann cells were labeled with a rabbit polyclonal anti-nestin (SCRR-1001, at 1:200; American Type Culture Collection, Manassas, VA) and anti-rabbit secondary antibody conjugated to TRITC (55671, at 1:400; Cappel).

Techniques: Mutagenesis, Labeling

Journal: The Journal of Neuroscience

Article Title: Induction of Neuregulin Signaling in Mouse Schwann Cells In Vivo Mimics Responses to Denervation

doi: 10.1523/JNEUROSCI.1086-06.2006

Figure Lengend Snippet: Induction of active ErbB2 results in upregulation of nestin expression in tSCs. Active ErbB2 was induced in SCs in an adult mouse by 7 d of Dox administration. A, SCs visualized by the transgenic expression of GFP. B, Nestin visualized by application of a polyclonal antibody and a rhodamine secondary. C, Axons visualized by the transgenic expression of CFP. D, AChRs visualized by application of Alexa 647–bungarotoxin. The nestin label is present in tSCs present at the junction and in processes extended by them (arrow in A, B); one of these processes is clearly associated with a nerve sprout (asterisks in C). Nestin label is also present in an extrajunctional SC (B, arrowhead). Scale bar, 30 μm.

Article Snippet: Reactive Schwann cells were labeled with a rabbit polyclonal anti-nestin (SCRR-1001, at 1:200; American Type Culture Collection, Manassas, VA) and anti-rabbit secondary antibody conjugated to TRITC (55671, at 1:400; Cappel).

Techniques: Expressing, Transgenic Assay